CD8 Binding of MHC-Peptide Complexes in cis or trans Regulates CD8<sup>+</sup> T-cell Responses
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00517958" target="_blank" >RIV/61388955:_____/19:00517958 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0303171" target="_blank" >http://hdl.handle.net/11104/0303171</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jmb.2019.10.019" target="_blank" >10.1016/j.jmb.2019.10.019</a>
Alternative languages
Result language
angličtina
Original language name
CD8 Binding of MHC-Peptide Complexes in cis or trans Regulates CD8<sup>+</sup> T-cell Responses
Original language description
The coreceptor CD8αβ can greatly promote activation of T cells by strengthening T-cell receptor (TCR) binding to cognate peptide-MHC complexes (pMHC) on antigen presenting cells and by bringing p56Lck to TCR/CD3. Here, we demonstrate that CD8 can also bind to pMHC on the T cell (in cis) and that this inhibits their activation. Using molecular modeling, fluorescence resonance energy transfer experiments on living cells, biochemical and mutational analysis, we show that CD8 binding to pMHC in cis involves a different docking mode and is regulated by posttranslational modifications including a membrane-distal interchain disulfide bond and negatively charged O-linked glycans near positively charged sequences on the CD8β stalk. These modifications distort the stalk, thus favoring CD8 binding to pMHC in cis. Differential binding of CD8 to pMHC in cis or trans is a means to regulate CD8+ T-cell responses and provides new translational opportunities.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Molecular Biology
ISSN
0022-2836
e-ISSN
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Volume of the periodical
431
Issue of the periodical within the volume
24
Country of publishing house
GB - UNITED KINGDOM
Number of pages
18
Pages from-to
4941-4958
UT code for WoS article
000506722000013
EID of the result in the Scopus database
2-s2.0-85075826097