Different Mechanisms of DNA Radiosensitization by 8-Bromoadenosine and 2 '-Deoxy-2 '-fluorocytidine Observed on DNA Origami Nanoframe Supports

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F22%3A00558117" target="_blank" >RIV/61388955:_____/22:00558117 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/22:00558117 RIV/61389005:_____/22:00558117
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.jpclett.2c00584" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.jpclett.2c00584</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.jpclett.2c00584" target="_blank" >10.1021/acs.jpclett.2c00584</a>

Result language
angličtina
Original language name
Different Mechanisms of DNA Radiosensitization by 8-Bromoadenosine and 2 '-Deoxy-2 '-fluorocytidine Observed on DNA Origami Nanoframe Supports
Original language description
DNA origami nanoframes with two parallel DNA sequences are used to evaluate the effect of nucleoside substituents on radiation-induced DNA damage. Double strand breaks (DSB) of DNA are counted using atomic force microscopy (AFM), and total number of lesions is evaluated using real-time polymerase chain reaction (RT-PCR). Enhanced AT or GC content does not increase the number of DNA strand breaks. Incorporation of 8-bromoadenosine results in the highest enhancement in total number of lesions. However, the highest enhancement in DSB is observed for 2´-deoxy-2´-fluorocytidine, indicating different mechanisms of radiosensitization by nucleoside analogues with the halogen substituent on base or sugar moieties, respectively. ´´Bystander´´ effects are observed, when the number of DSB in a sequence is enhanced by a substituent in the parallel DNA sequence. The present approach eliminates limitations of previously developed methods and motivates detailed studies of poorly understood conformation or bystander effects in radiation induced damage to DNA.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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