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Light-induced damage to DNA origami nanostructures in the 193 nm-310 nm range

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00575871" target="_blank" >RIV/61388955:_____/23:00575871 - isvavai.cz</a>

  • Alternative codes found

    RIV/46747885:24510/23:00012257

  • Result on the web

    <a href="https://hdl.handle.net/11104/0345575" target="_blank" >https://hdl.handle.net/11104/0345575</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1088/1361-6455/acf3bd" target="_blank" >10.1088/1361-6455/acf3bd</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Light-induced damage to DNA origami nanostructures in the 193 nm-310 nm range

  • Original language description

    DNA origami nanostructures provide precisely addressable substrates for in singulo experiments as well as for applications in nanotechnology. We report on experiments evaluating the stability of DNA origami upon irradiation with light at different wavelengths and buffer solutions. DNA is irradiated with nanosecond pulsed lasers and the damage is evaluated using UV-Vis spectroscopy and atomic force microscopy imaging. We show that the wavelength dependence of the damage follows the UV absorption spectrum of DNA. Electronic excitation of DNA is primarily responsible for DNA origami damage at present wavelengths. We also demonstrate UV-Vis absorption of tris reaction products, influencing the UV-Vis absorption evaluation in experiments studying DNA damage.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10403 - Physical chemistry

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Physics B-Atomic Molecular and Optical Physics

  • ISSN

    0953-4075

  • e-ISSN

    1361-6455

  • Volume of the periodical

    56

  • Issue of the periodical within the volume

    18

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    185101

  • UT code for WoS article

    001059517700001

  • EID of the result in the Scopus database

    2-s2.0-85171023334