First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00576143" target="_blank" >RIV/61388955:_____/23:00576143 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10472904
Result on the web
<a href="https://hdl.handle.net/11104/0345735" target="_blank" >https://hdl.handle.net/11104/0345735</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.microc.2023.109403" target="_blank" >10.1016/j.microc.2023.109403</a>

Result language
angličtina
Original language name
First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6
Original language description
A novel electrochemical bioplatform for Pru du 6 determination, one of the most abundant and allergenic almond proteins, was developed to detect traces of almonds in processed foods. The bioplatform combines the application of a sandwich immunoassay on the surface of magnetic beads using specific antibodies unmodified or conjugated to horseradish peroxidase as capture and detection antibodies. The resulting magnetic bioconjugates are trapped on the surface of disposable carbon electrodes, and amperometry in the presence of hydrogen peroxide and hydroquinone is employed to measure the change in the cathodic current, which is proportional to Pru du 6 concentration. The developed bioplatform exhibited good selectivity and sensitivity, offering a detection limit of 0.12 ng mL−1 of Pru du 6. It could determine Pru du 6 in raw dough and baked cookies incurred with 0.2 µg g−1 of almond protein with acceptable precision, providing results comparable to those obtained using an ELISA technique.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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