Electrochemical immunosensing of walnut and hazelnut allergenic proteins in processed foods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F24%3A00585010" target="_blank" >RIV/61388955:_____/24:00585010 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10487999

Result on the web

<a href="https://hdl.handle.net/11104/0352781" target="_blank" >https://hdl.handle.net/11104/0352781</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sbsr.2024.100644" target="_blank" >10.1016/j.sbsr.2024.100644</a>

Result language

angličtina

Original language name

Electrochemical immunosensing of walnut and hazelnut allergenic proteins in processed foods

Original language description

Nuts are a well-known cause of food allergy and, once this has been diagnosed, due to the likelihood of cross-sensitization to multiple tree nut allergens, their strict avoidance from the diet is advisable. In this context, we present electrochemical bioplatforms to detect traces of hazelnut and walnut in processed foods through the determination of their respective allergenic proteins Cor a 9 and Jug r 1 in a fast and sensitive assay. First, the evaluation of the single determination of both proteins was performed by building sandwich immunoconjugates on the surface of magnetic microbeads relying on specific antibodies unmodified or conjugated to horseradish peroxidase. Amperometric transduction was made upon trapping the magnetic bioconjugates on the surface of disposable carbon electrodes, using the hydroquinone/hydrogen peroxide system. The great analytical performance achieved with the individual platforms (detection limits of 0.12 and 0.56 ng mL−1 for Jug r 1 and Cor a 9, respectively), led us to the individual and dual quantification of both proteins in raw dough and baked cookies incurred with ground nuts. The developed method allowed detecting baked cookies incurred with 0.0025% ground walnut and 0.00002% ground hazelnut with results comparable to those provided by ELISA techniques. The feasibility of performing the dual determination of both allergens in a single run was demonstrated.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensing and Bio-Sensing Research

ISSN

2214-1804

e-ISSN

2214-1804

Volume of the periodical

44

Issue of the periodical within the volume

JUN 2024

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

100644

UT code for WoS article

001289802900001

EID of the result in the Scopus database

2-s2.0-85189086393