CpG Methylation Protects DNA against Ionizing Radiation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F25%3A00638734" target="_blank" >RIV/61388955:_____/25:00638734 - isvavai.cz</a>
Alternative codes found
RIV/61389005:_____/25:00638680
Result on the web
<a href="https://hdl.handle.net/11104/0369324" target="_blank" >https://hdl.handle.net/11104/0369324</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.jpcb.5c04043" target="_blank" >10.1021/acs.jpcb.5c04043</a>
Alternative languages
Result language
angličtina
Original language name
CpG Methylation Protects DNA against Ionizing Radiation
Original language description
Methylation of DNA CpG domains in cellular DNA is a key mechanism of epigenetic regulation. Disruptions in the processes maintaining DNA methylation can lead to diseases like cancer. The radiation response of certain cancer cells may be affected by their DNA methylation levels, which may have consequences in their response to radiotherapy. In this work, we utilized DNA origami nanotechnology to examine whether DNA methylation impacts DNA response to ionizing radiation in solution before biological processes come into play. Our findings reveal that a protective effect is achieved with just a few methylated CpG adducts. Both low-LET (electron) and high-LET (carbon ion) irradiation show a reduced lesion count in methylated DNA, as indicated by qPCR results. AFM single-molecule observations using DNA origami nanoframes suggest fewer double-strand breaks in methylated DNA after carbon ion irradiation. This radioprotective effect may contribute to the differential radiation response of cellular DNA and should be considered when predicting and evaluating DNA radiation damage yields.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/EH22_008%2F0004558" target="_blank" >EH22_008/0004558: Advanced MUltiscaLe materials for key Enabling Technologies</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Physical Chemistry B
ISSN
1520-6106
e-ISSN
1520-5207
Volume of the periodical
129
Issue of the periodical within the volume
35
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
8880-8887
UT code for WoS article
001554780100001
EID of the result in the Scopus database
2-s2.0-105015417545