New analogs of the CART peptide with anorexigenic potency: The importance of individual disulfide bridges
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00391965" target="_blank" >RIV/61388963:_____/13:00391965 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.peptides.2012.09.033" target="_blank" >http://dx.doi.org/10.1016/j.peptides.2012.09.033</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.peptides.2012.09.033" target="_blank" >10.1016/j.peptides.2012.09.033</a>
Alternative languages
Result language
angličtina
Original language name
New analogs of the CART peptide with anorexigenic potency: The importance of individual disulfide bridges
Original language description
The CART (cocaine- and amphetamine-regulated transcript) peptide is an anorexigenic neuropeptide that acts in the hypothalamus. The receptor and the mechanism of action of this peptide are still unknown. In our previous study, we showed that the CART peptide binds specifically to PC12 rat pheochromocytoma cells in both the native and differentiated into neuronal phenotype. Two biologically active forms, CART(55-102) and CART(61-102), with equal biological activity, contain three disulfide bridges. To clarify the importance of each of these disulfide bridges in maintaining the biological activity of CART(61-102), an Ala scan at particular S-S bridges forming cysteines was performed, and analogs with only one or two disulfide bridges were synthesized. Inthis study, a stabilized CART(61-102) analog with norleucine instead of methionine at position 67 was also prepared and was found to bind to PC12 cells with an anorexigenic potency similar to that of CART(61-102). The binding study revea
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP303%2F10%2F1368" target="_blank" >GAP303/10/1368: New pharmacological interventions influencing energy homeostasis and development of insulin resistance/ type 2 diabetes mellitus</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Peptides
ISSN
0196-9781
e-ISSN
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Volume of the periodical
39
Issue of the periodical within the volume
January
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
138-144
UT code for WoS article
000315839300021
EID of the result in the Scopus database
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