Specific features of Zn2+, Co2+ and Ni2+ ion binding to DNA in alkaline solutions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00392688" target="_blank" >RIV/61388963:_____/13:00392688 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2013.01.001" target="_blank" >http://dx.doi.org/10.1016/j.ijbiomac.2013.01.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2013.01.001" target="_blank" >10.1016/j.ijbiomac.2013.01.001</a>

Result language
angličtina
Original language name
Specific features of Zn2+, Co2+ and Ni2+ ion binding to DNA in alkaline solutions
Original language description
Dependence of DNA metallization degree during B-DNA transition into the metallized (m) form on DNA concentration has been studied by visible and differential UV-spectroscopy in the presence of Zn2+, Co2+ and Ni2+ ions in tetraborate buffer (pH 8.5) withand without ethidium bromide. Constants of Mt(2+) binding to double stranded DNA were calculated. The obtained binding constants corresponded to the formation of inter-strand metal bridges stabilizing m-form. Thermodynamic origin of higher efficiency ofZn2+ ions in DNA metallization compared to Co2+ and Ni2+ was revealed. Increase of the DNA helix-coil transition temperature by up to 10 degrees C upon formation of m-form in the presence of Zn2+ ions was observed and rationalized. Furthermore, a strongcooperative decrease (up to 30 degrees C) of the temperature of B m transition induced by heating in the presence of Zn2+ was found and its nature was explained.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/GAP208%2F10%2F0559" target="_blank" >GAP208/10/0559: Theoretical and spectroscopic studies of nucleic acid self-assemblies</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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