DNA conformational equilibrium in the presence of Zn2+ ions in neutral and alkaline solutions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00376575" target="_blank" >RIV/61388963:_____/12:00376575 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2011.11.011" target="_blank" >http://dx.doi.org/10.1016/j.ijbiomac.2011.11.011</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2011.11.011" target="_blank" >10.1016/j.ijbiomac.2011.11.011</a>

Result language
angličtina
Original language name
DNA conformational equilibrium in the presence of Zn2+ ions in neutral and alkaline solutions
Original language description
Effect of Zn2+ ions on DNA transition from B-form to a metallized form (m-DNA) in Tris and tetraborate buffers at pH 8.5 has been studied by visible and differential UV-spectroscopy and by thermal denaturation. The results have been compared to those obtained at pH 6.5 in cacodylate buffer. It was found that in alkaline solutions Zn2+ ions induced a hypochromicity of the DNA absorption in the whole spectral range monitored, which was attributed to DNA transition from B- to the m-form. Complete metallization occurred only upon heating the DNA solutions containing more than similar to 2 x 10(-4) M of Zn2+ ions. Phase diagrams of the DNA-zinc complexes at pH 6.5 and 8.5 have been obtained for the first time. The m-DNA form showed higher thermal stabilitycompared to B-DNA.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
—

Project
<a href="/en/project/GAP208%2F10%2F0559" target="_blank" >GAP208/10/0559: Theoretical and spectroscopic studies of nucleic acid self-assemblies</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)