Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00420878" target="_blank" >RIV/61388963:_____/13:00420878 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2013.08.010" target="_blank" >http://dx.doi.org/10.1016/j.ijbiomac.2013.08.010</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2013.08.010" target="_blank" >10.1016/j.ijbiomac.2013.08.010</a>

Result language
angličtina
Original language name
Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions
Original language description
Effect of Zn2+ ions on the conformation of polyA in cacodilic buffer at pH 7 was investigated by differential UV spectroscopy (DUV) and by thermal denaturation. The shapes of the DUV spectra and melting curves suggest a transition of polyA into a more ordered "metallized", possibly double-helical conformation at Zn2+ concentrations above 3 x 10(-5) M. A phase diagram of polyA complexes with Zn2+ was constructed for the temperature range from 20 degrees C to 95 degrees C and Zn2+ concentrations between 10(-5) M and 5 x 10(-4) M. It was found that the transition of a single strand into the "metallized" form is possible only if the length of the disordered single-stranded region becomes larger than a certain critical value, ranging between 98% and 78% asthe metal concentration increases from 3 x 10(-5) to 5 x 10(-4) M.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
<a href="/en/project/GAP208%2F10%2F0559" target="_blank" >GAP208/10/0559: Theoretical and spectroscopic studies of nucleic acid self-assemblies</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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