Divalent metal ion effect on helix-coil transition of high molecular weight DNA in neutral and alkaline solutions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F11%3A00361375" target="_blank" >RIV/61388963:_____/11:00361375 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2010.12.016" target="_blank" >http://dx.doi.org/10.1016/j.ijbiomac.2010.12.016</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2010.12.016" target="_blank" >10.1016/j.ijbiomac.2010.12.016</a>
Alternative languages
Result language
angličtina
Original language name
Divalent metal ion effect on helix-coil transition of high molecular weight DNA in neutral and alkaline solutions
Original language description
Effect of Mg2+, Ca2+, Ni2+ and Cd2+ ions on parameters of DNA helix-coil transition in sodium cacodylate (pH 6.5), Tris (pH 8.5) and sodium tetraborate (pH 9.0) buffers have been studied by differential UV-visible spectroscopy and thermal denaturation. Anomalous behavior of the melting temperature Tm and the melting interval .delta.? in the presence of MgCl2 was observed in Tris, but not in cacodylate or tetraborate buffers. Decrease of the Tm and .delta.? of DNA in the presence of Ni2+ and Cd2+ was caused by preferential ion interaction with N7 of guanine. This type of interaction was also found for Mg2+ in Tris buffer. The anomalous decrease in the Tm and .delta.? values was connected to formation of complexes between metal ions and Tris molecules.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP208%2F10%2F0559" target="_blank" >GAP208/10/0559: Theoretical and spectroscopic studies of nucleic acid self-assemblies</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Biological Macromolecules
ISSN
0141-8130
e-ISSN
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Volume of the periodical
48
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
369-374
UT code for WoS article
000288408500022
EID of the result in the Scopus database
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