N-Branched acyclic nucleoside phosphonates as monomers for the synthesis of modified oligonucleotides

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00444054" target="_blank" >RIV/61388963:_____/15:00444054 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1039/c4ob02265c" target="_blank" >http://dx.doi.org/10.1039/c4ob02265c</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c4ob02265c" target="_blank" >10.1039/c4ob02265c</a>

Result language

angličtina

Original language name

N-Branched acyclic nucleoside phosphonates as monomers for the synthesis of modified oligonucleotides

Original language description

Protected N-branched nucleoside phosphonates containing adenine and thymine bases were prepared as the monomers for the introduction of aza-acyclic nucleotide units into modified oligonucleotides. The phosphotriester and phosphoramidite methods were usedfor the incorporation of modified and natural units, respectively. The solid phase synthesis of a series of nonamers containing one central modified unit was successfully performed in both 3'-> 5' and 5'-> 3' directions. Hybridization properties of theprepared oligoribonucleotides and oligodeoxyribonucleotides were evaluated. The measurement of thermal characteristics of the complexes of modified nonamers with the complementary strand revealed a considerable destabilizing effect of the introduced units. We also examined the substrate/inhibitory properties of aza-acyclic nucleoside phosphono-diphosphate derivatives (analogues of nucleoside triphosphates) but neither inhibition of human and bacterial DNA polymerases nor polymerase-media

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CC - Organic chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Organic & Biomolecular Chemistry

ISSN

1477-0520

e-ISSN

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Volume of the periodical

13

Issue of the periodical within the volume

15

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

4449-4458

UT code for WoS article

000352546100013

EID of the result in the Scopus database

2-s2.0-84926512826