Cyclodextrin-peptide conjugates for sequence specific DNA binding

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00444387" target="_blank" >RIV/61388963:_____/15:00444387 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/c5ob00609k" target="_blank" >http://dx.doi.org/10.1039/c5ob00609k</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c5ob00609k" target="_blank" >10.1039/c5ob00609k</a>

Result language
angličtina
Original language name
Cyclodextrin-peptide conjugates for sequence specific DNA binding
Original language description
Synthetic models of bZIP transcription factors have been developed with the capability of specific DNA recognition. Our design is based on the CuAAC mediated conjugation of basic region Leucine Zipper peptides to different derivatives of alpha,beta and gamma-cyclodextrins equipped with azide functionalities. Thorough optimization of reaction conditions allowed convergent and simultaneous conjugation of two long unprotected cationic peptides to cyclodextrin-bis azide derivatives. The resulting constructswere shown to specifically recognize their cognate DNA sequence with nM affinities. In comparison with previously developed TF models, the derivatives described here combine the enhanced DNA binding capabilities with an easy and convergent synthetic route.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
—

Project
<a href="/en/project/LD12019" target="_blank" >LD12019: Dynamic libraries of cyclodextrin duplexes as a source of high affinity hosts for complexation of organic molecules in aqueous environment.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)