Estimation of apparent binding constant of complexes of selected acyclic nucleoside phosphonates with beta-cyclodextrin by affinity capillary electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00458609" target="_blank" >RIV/61388963:_____/16:00458609 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201500337" target="_blank" >http://dx.doi.org/10.1002/elps.201500337</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201500337" target="_blank" >10.1002/elps.201500337</a>
Alternative languages
Result language
angličtina
Original language name
Estimation of apparent binding constant of complexes of selected acyclic nucleoside phosphonates with beta-cyclodextrin by affinity capillary electrophoresis
Original language description
Affinity capillary electrophoresis (ACE) has been applied to estimation of apparent binding constant of complexes of (R,S)-enantiomers of selected acyclic nucleoside phosphonates (ANPs) with chiral selector beta-cyclodextrin (beta CD) in aqueous alkaline medium. The non-covalent interactions of five pairs of (R,S)-enantiomers of ANPs-based antiviral drugs and their derivatives with beta CD were investigated in the background electrolyte (BGE) composed of 35 or 50 mM sodium tetraborate, pH 10.0, and containing variable concentration (0-25 mM) of beta CD. The apparent binding constants of the complexes of (R,S)-enantiomers of ANPs with CD were estimated from the dependence of effective electrophoretic mobilities of (R,S)-enantiomers of ANPs (measured simultaneously by ACE at constant reference temperature 25 degrees C inside the capillary) on the concentration of beta CD in the BGE using different nonlinear and linear calculation methodologies. Nonlinear regression analysis provided more precise and accurate values of the binding constants and a higher correlation coefficient as compared to the regression analysis of the three linearized plots of the effective mobility dependence on beta CD concentration in the BGE. The complexes of (R,S)-enantiomers of ANPs with beta CD have been found to be relatively weak - their apparent binding constants determined by the nonlinear regression analysis were in the range 13.3-46.4 L/mol whereas the values from the linearized plots spanned the interval 12.3-55.2 L/mol.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
37
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
239-247
UT code for WoS article
000368030000002
EID of the result in the Scopus database
2-s2.0-84953726061