Affinity Capillary Electrophoresis Applied to Investigation of Valinomycin Complexes with Ammonium and Alkali Metal Ions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00467288" target="_blank" >RIV/61388963:_____/16:00467288 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-4939-4014-1_17" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-4014-1_17</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-4014-1_17" target="_blank" >10.1007/978-1-4939-4014-1_17</a>
Alternative languages
Result language
angličtina
Original language name
Affinity Capillary Electrophoresis Applied to Investigation of Valinomycin Complexes with Ammonium and Alkali Metal Ions
Original language description
This chapter deals with the application of affinity capillary electrophoresis (ACE) to investigation of noncovalent interactions (complexes) of valinomycin, a macrocyclic dodecadepsipeptide antibiotic ionophore, with ammonium and alkali metal ions (lithium, sodium, potassium, rubidium, and cesium). The strength of these interactions was characterized by the apparent binding (stability, association) constants (K-b) of the above valinomycin complexes using the mobility shift assay mode of ACE. The study involved measurements of effective electrophoretic mobility of valinomycin at variable concentrations of ammonium or alkali metal ions in the background electrolyte (BGE). The effective electrophoretic mobilities of valinomycin measured at ambient temperature and variable ionic strength were first corrected to the reference temperature 25 degrees C and constant ionic strength (10 or 25 mM). Then, from the dependence of the corrected valinomycin effective mobility on the ammonium or alkali metal ion concentration in the BGE, the apparent binding constants of the valinomycin-ammonium or valinomycin-alkali metal ion complexes were determined using a nonlinear regression analysis. Logarithmic form of the binding constants (log Kb) were found to be in the range of 1.50-4.63, decreasing in the order Rb+ > K+ > Cs+ > > Na+ > NH4+ similar to Li+.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols
ISBN
978-1-4939-4012-7
Number of pages of the result
14
Pages from-to
219-232
Number of pages of the book
234
Publisher name
Springer
Place of publication
New York
UT code for WoS chapter
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