Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00509114" target="_blank" >RIV/61388963:_____/19:00509114 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41330/19:81523
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900043" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900043</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201900043" target="_blank" >10.1002/elps.201900043</a>
Alternative languages
Result language
angličtina
Original language name
Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol
Original language description
Affinity capillary electrophoresis (ACE) and pressure-assisted ACE were employed to study the noncovalent molecular interactions of antamanide (AA), cyclic decapeptide from the deadly poisonous fungus Amanita phalloides, with univalent (Li+, Na+, K+, and NH4+) and divalent (Mg2+ and Ca2+) cations in methanol. The strength of these interactions was quantified by the apparent stability constants of the appropriate AA-cation complexes. The stability constants were calculated using the nonlinear regression analysis of the dependence of the effective electrophoretic mobility of AA on the concentration of the above ions in the BGE (methanolic solution of 20 mM chloroacetic acid, 10 mM Tris, pH(MeOH) 7.8, containing 0-50 mM concentrations of the above ions added in the form of chlorides). Prior to stability constant calculation, the AA effective mobilities measured at actual temperature inside the capillary and at variable ionic strength of the BGEs were corrected to the values corresponding to the reference temperature of 25 degrees C and to the constant ionic strength of 10 mM. From the above ions, sodium cation interacted with AA moderately strong with the stability constant 362 +/- 16 L/mol. K+, Mg2+, and Ca2+ cations formed with AA weak complexes with stability constants in the range 37-31 L/mol decreasing in the order K+ > Ca2+ > Mg2+. No interactions were observed between AA and small Li+ and large NH4+ cations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
40
Issue of the periodical within the volume
18/19
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
2321-2328
UT code for WoS article
000483858400010
EID of the result in the Scopus database
2-s2.0-85065993665