Affinity capillary electrophoresis and quantum mechanical calculations applied to investigation of [Gly(6)]-antamanide binding with sodium and potassium ions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00477160" target="_blank" >RIV/61388963:_____/17:00477160 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41330/17:74584
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201600474" target="_blank" >http://dx.doi.org/10.1002/elps.201600474</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201600474" target="_blank" >10.1002/elps.201600474</a>
Alternative languages
Result language
angličtina
Original language name
Affinity capillary electrophoresis and quantum mechanical calculations applied to investigation of [Gly(6)]-antamanide binding with sodium and potassium ions
Original language description
ACE in a free solution and quantum mechanical density functional theory have been applied to the investigation of interactions of glycine-6-antamanide ([Gly(6)]AA), a synthetic derivative of cyclic decapeptide antamanide isolated from the highly poisonous mushroom Amanita phalloides, with sodium or potassium ions in methanol. First, from the dependence of effective electrophoretic mobility of [Gly(6)]AA on Na+ or K+ ions concentration in the BGE (methanolic solution of 20 mM chloroacetic acid, 10 mM Tris, pH(MeOH) 7.8, containing 0-50 mM NaCl or 0-40 mM KCl), the apparent binding (stability) constants of [Gly(6)]AA-Na+ and [Gly(6)]AA-K+ complexes were evaluated as 26 +/- 1 and 14 +/- 1L/mol, respectively. The employed ACE method included correction of the effective mobilities measured at ambient temperature and at variable ionic strength of the BGEs to the mobilities related to the reference temperature 25 degrees C and to the constant ionic strength 10 mM. Second, the interaction energies of the [Gly(6)]AA-Na+ and [Gly(6)]AA-K+ complexes (-466.3 and -345.2 kJ/mol, respectively) and the structural details of these complexes, such as position of the Na+ and K+ ions in the cavity of the [Gly(6)]AA molecule and the interatomic distances within these complexes, were determined by the density functional theory calculations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA15-01948S" target="_blank" >GA15-01948S: Capillary electromigration techniques using affinity selectors & smart polymers for analysis and properties and interactions studies of biomolecules</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
38
Issue of the periodical within the volume
12
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
1551-1559
UT code for WoS article
000403912000003
EID of the result in the Scopus database
2-s2.0-85013188067