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Inhibition of non-templated nucleotide addition by DNA polymerases in primer extension using twisted intercalating nucleic acid modified templates

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00458681" target="_blank" >RIV/61388963:_____/16:00458681 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/16:10317125

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.bmcl.2015.12.034" target="_blank" >http://dx.doi.org/10.1016/j.bmcl.2015.12.034</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.bmcl.2015.12.034" target="_blank" >10.1016/j.bmcl.2015.12.034</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Inhibition of non-templated nucleotide addition by DNA polymerases in primer extension using twisted intercalating nucleic acid modified templates

  • Original language description

    A simple and elegant method for inhibition of non-templated nucleotide addition by DNA polymerases and for following DNA 30-heterogeneity in enzymatic DNA synthesis by primer extension (PEX) is described. When template bearing ortho-twisted intercalating nucleic acid (ortho-TINA) at the 5'-end is used, non-templated nucleotide addition is reduced in both the A-and B-family DNA polymerases (KOD XL, KOD (exo-), Bst 2.0, Therminator, Deep Vent (exo-) and Taq). Formation of a single oligonucleotide product was observed with ortho-TINA modified template and KOD XL, KOD (exo-), Bst 2.0, Deep Vent (exo-) and Taq DNA polymerases. This approach can be applied to the synthesis of both unmodified and base-modified oligonucleotides.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CC - Organic chemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Bioorganic and Medicinal Chemistry Letters

  • ISSN

    0960-894X

  • e-ISSN

  • Volume of the periodical

    26

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    4

  • Pages from-to

    288-291

  • UT code for WoS article

    000367329700009

  • EID of the result in the Scopus database

    2-s2.0-84952861737