Determination of effective charges and ionic mobilities of polycationic antimicrobial peptides by capillary isotachophoresis and capillary zone electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00478419" target="_blank" >RIV/61388963:_____/17:00478419 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201700092" target="_blank" >http://dx.doi.org/10.1002/elps.201700092</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201700092" target="_blank" >10.1002/elps.201700092</a>
Alternative languages
Result language
angličtina
Original language name
Determination of effective charges and ionic mobilities of polycationic antimicrobial peptides by capillary isotachophoresis and capillary zone electrophoresis
Original language description
Capillary ITP (CITP) and CZE were applied to the determination of effective charges and ionic mobilities of polycationic antimicrobial peptides (AMPs). Twelve AMPs (deca- to hexadecapeptides) containing three to seven basic amino acid residues (His, Lys, Arg) at variable positions of peptide chain were investigated. Effective charges of the AMPs were determined from the lengths of their ITP zones, ionic mobilities, and molar concentrations, and from the same parameters of the reference compounds. Lengths of the ITP zones of AMPs and reference compounds were obtained from their CITP analyses in cationic mode using leading electrolyte (LE) composed of 10mMNH(4)OH, 40mMAcOH (acetic acid), pH 4.1, and terminating electrolyte (TE) containing 40mMAcOH, pH 3.2. Ionic mobilities of AMPs and singly charged reference compounds (ammediol or arginine) were determined by their CZE analyses in the BGE of the same composition as the LE. The effective charges numbers of AMPs were found to be in the range 1.65-5.04, i.e. significantly reduced as compared to the theoretical charge numbers (2.86-6.99) calculated from the acidity constants of the analyzed AMPs. This reduction of effective charge due to tightly bound acetate counterions (counterion condensation) was in the range 17-47% depending on the number and type of the basic amino acid residues in the AMPs molecules. Ionic mobilities of AMPs achieved values (26.5-38.6)x10(-9)m(2)V(-1)s(-1) and in most cases were in a good agreement with the ratio of their effective charges and relative molecular masses.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA15-01948S" target="_blank" >GA15-01948S: Capillary electromigration techniques using affinity selectors & smart polymers for analysis and properties and interactions studies of biomolecules</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
38
Issue of the periodical within the volume
16
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
2018-2024
UT code for WoS article
000408038500006
EID of the result in the Scopus database
2-s2.0-85019765806