Novel (p)ppGpp Binding and Metabolizing Proteins of Escherichia coli

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00490330" target="_blank" >RIV/61388963:_____/18:00490330 - isvavai.cz</a>

Result on the web

<a href="http://mbio.asm.org/content/9/2/e02188-17.full" target="_blank" >http://mbio.asm.org/content/9/2/e02188-17.full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/mBio.02188-17" target="_blank" >10.1128/mBio.02188-17</a>

Result language

angličtina

Original language name

Novel (p)ppGpp Binding and Metabolizing Proteins of Escherichia coli

Original language description

The alarmone (p) ppGpp plays pivotal roles in basic bacterial stress responses by increasing tolerance of various nutritional limitations and chemical insults, including antibiotics. Despite intensive studies since (p) ppGpp was discovered over 4 decades ago, (p) ppGpp binding proteins have not been systematically identified in Escherichia coli. We applied DRaCALA (differential radial capillary action of ligand assay) to identify (p) ppGpp-protein interactions. We discovered 12 new (p) ppGpp targets in E. coli that, based on their physiological functions, could be classified into four major groups, involved in (i) purine nucleotide homeostasis (YgdH), (ii) ribosome biogenesis and translation (RsgA, Era, HflX, and LepA), (iii) maturation of dehydrogenases (HypB), and (iv) metabolism of (p) ppGpp (MutT, NudG, TrmE, NadR, PhoA, and UshA). We present a comprehensive and comparative biochemical and physiological characterization of these novel (p) ppGpp targets together with a comparative analysis of relevant, known (p) ppGpp binding proteins. Via this, primary targets of (p) ppGpp in E. coli are identified. The GTP salvage biosynthesis pathway and ribosome biogenesis and translation are confirmed as targets of (p) ppGpp that are highly conserved between E. coli and Firmicutes. In addition, an alternative (p) ppGpp degradative pathway, involving NudG and MutT, was uncovered. This report thus significantly expands the known cohort of (p) ppGpp targets in E. coli.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA15-11711S" target="_blank" >GA15-11711S: Development of a molecular toolkit for control and investigation of the bacterial stringent response</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

mBio

ISSN

2150-7511

e-ISSN

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Volume of the periodical

9

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

20

Pages from-to

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UT code for WoS article

000431279600021

EID of the result in the Scopus database

2-s2.0-85046494288