Identification of a molecular gating determinant within the carboxy terminal region of Ca(v)3.3 T-type channels

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00504415" target="_blank" >RIV/61388963:_____/19:00504415 - isvavai.cz</a>

Result on the web

<a href="https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-019-0457-0" target="_blank" >https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-019-0457-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13041-019-0457-0" target="_blank" >10.1186/s13041-019-0457-0</a>

Result language

angličtina

Original language name

Identification of a molecular gating determinant within the carboxy terminal region of Ca(v)3.3 T-type channels

Original language description

The physiological functions controlled by T-type channels are intrinsically dependent on their gating properties, and alteration of T-type channel activity is linked to several human disorders. Therefore, it is essential to develop a clear understanding of the structural determinants responsible for the unique gating features of T-type channels. Here, we have investigated the specific role of the carboxy terminal region by creating a series a deletion constructs expressed in tsA-201 cells and analyzing them by patch clamp electrophysiology. Our data reveal that the proximal region of the carboxy terminus contains a structural determinant essential for shaping several gating aspects of Ca(v)3.3 channels, including voltage-dependence of activation and inactivation, inactivation kinetics, and coupling between the voltage sensing and the pore opening of the channel. Altogether, our data are consistent with a model in which the carboxy terminus stabilizes the channel in a closed state.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Brain

ISSN

1756-6606

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

Apr 8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

34

UT code for WoS article

000464218100002

EID of the result in the Scopus database

2-s2.0-85064120593