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Functional Characterization of Secreted Aspartyl Proteases in Candida parapsilosis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00508859" target="_blank" >RIV/61388963:_____/19:00508859 - isvavai.cz</a>

  • Result on the web

    <a href="https://msphere.asm.org/content/4/4/e00484-19" target="_blank" >https://msphere.asm.org/content/4/4/e00484-19</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1128/mSphere.00484-19" target="_blank" >10.1128/mSphere.00484-19</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Functional Characterization of Secreted Aspartyl Proteases in Candida parapsilosis

  • Original language description

    Candida parapsilosis is an emerging non-albicans Candida species that largely affects low-birth-weight infants and immunocompromised patients. Fungal pathogenesis is promoted by the dynamic expression of diverse virulence factors, with secreted proteolytic enzymes being linked to the establishment and progression of disease. Although secreted aspartyl proteases (Sap) are critical for Candida albicans pathogenicity, their role in C. parapsilosis is poorly elucidated. In the present study, we aimed to examine the contribution of C. parapsilosis SAPP genes SAPP1, SAPP2, and SAPP3 to the virulence of the species. Our results indicate that SAPP1 and SAPP2, but not SAPP3, influence adhesion, host cell damage, phagosomelysosome maturation, phagocytosis, killing capacity, and cytokine secretion by human peripheral blood-derived macrophages. Purified Sapp1p and Sapp2p were also shown to efficiently cleave host complement component 3b (C3b) and C4b proteins and complement regulator factor H. Additionally, Sapp2p was able to cleave factor H-related protein 5 (FHR-5). Altogether, these data demonstrate the diverse, significant contributions that SAPP1 and SAPP2 make to the establishment and progression of disease by C. parapsilosis through enabling the attachment of the yeast cells to mammalian cells and modulating macrophage biology and disruption of the complement cascade.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/LO1302" target="_blank" >LO1302: InterBioMed</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    mSphere

  • ISSN

    2379-5042

  • e-ISSN

  • Volume of the periodical

    4

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    16

  • Pages from-to

    e00484-19

  • UT code for WoS article

    000483320200054

  • EID of the result in the Scopus database

    2-s2.0-85071292702