Influence of cell-penetrating peptides on the activity and stability of virus-based nanoparticles

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00520889" target="_blank" >RIV/61388963:_____/20:00520889 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/20:10414055 RIV/00216208:11310/20:10414055

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0378517319310695?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378517319310695?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijpharm.2019.119008" target="_blank" >10.1016/j.ijpharm.2019.119008</a>

Result language

angličtina

Original language name

Influence of cell-penetrating peptides on the activity and stability of virus-based nanoparticles

Original language description

Viral nanoparticles represent potential natural versatile platforms for targeted gene and drug delivery. Improving the efficiency of gene transfer mediated by viral vectors could not only enhance their therapeutic potential, but also contribute to understanding the limitations in interactions of nanoparticles with cells and the development of new therapeutic approaches. In this study, four cell-penetrating peptides (CPPs), cationic octaarginine (R8), histidine-rich peptides (LAH4 and KH27K) and fusogenic peptide (FUSO), are investigated for their effect on infection by mouse polyomavirus (MPyV) or on transduction of reporter genes delivered by MPyV or related viral vectors. Peptides noncovalently associated with viral particles enhance gene transfer (with the exception of FUSO). Removal of cellular heparan sulfates by the heparinase does not significantly change the enhancing potential of CPPs. Instead, CPPs influences the physical state of viral particles: R8 slightly destabilizes the intact virus, KH27K induces its aggregation and LAH4 promotes disassembly and aggregation of the particles that massively and rapidly associate with cells. The findings indicate that peptides acting as transduction-enhancing agents of polyomavirus-based nanoparticles modulate their physical state, which can be an important prerequisite for sensitization of cells and determination of the further fate of viral particles inside cells.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10607 - Virology

Project

<a href="/en/project/GA17-11397S" target="_blank" >GA17-11397S: Study of the endocytotic machinery via modification of viral-based nanoparticles</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Pharmaceutics

ISSN

0378-5173

e-ISSN

—

Volume of the periodical

576

Issue of the periodical within the volume

Feb 25

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

119008

UT code for WoS article

000512972500031

EID of the result in the Scopus database

2-s2.0-85077643459