VirPorters: Insights into the action of cationic and histidine-rich cell-penetrating peptides

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00549351" target="_blank" >RIV/61388963:_____/22:00549351 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/22:10437084 RIV/00216208:11310/22:10437084

Result on the web

<a href="https://doi.org/10.1016/j.ijpharm.2021.121308" target="_blank" >https://doi.org/10.1016/j.ijpharm.2021.121308</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijpharm.2021.121308" target="_blank" >10.1016/j.ijpharm.2021.121308</a>

Result language

angličtina

Original language name

VirPorters: Insights into the action of cationic and histidine-rich cell-penetrating peptides

Original language description

The utilization of nanoparticles for the intracellular delivery of theranostic agents faces one substantial limitation. Sequestration in intracellular vesicles prevents them from reaching the desired location in the cytoplasm or nucleus to deliver their cargo. We investigated whether three different cell-penetrating peptides (CPPs), namely, octa-arginine R8, polyhistidine KH27K and histidine-rich LAH4, could promote cytosolic and/or nuclear transfer of unique model nanoparticles—pseudovirions derived from murine polyomavirus. Two types of CPP-modified pseudovirions that carry the luciferase reporter gene were created: VirPorters-IN with CPPs genetically attached to the capsid interior and VirPorters-EX with CPPs noncovalently associated with the capsid exterior. We tested their transduction ability by luciferase assay and monitored their presence in subcellular fractions. Our results confirmed the overall effect of CPPs on the intracellular destination of the particles and suggested that KH27K has the potential to improve the cytosolic release of pseudovirions. None of the VirPorters caused endomembrane damage detectable by the Galectin-3 assay. Remarkably, a noncovalent modification was required to promote high transduction of the reporter gene and cytosolic delivery of pseudovirions mediated by LAH4. Together, CPPs in different arrangements have demonstrated their potential to improve pseudovirion invasion into cells, and these findings could be useful for the development of other nanoparticle-based delivery systems.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA17-11397S" target="_blank" >GA17-11397S: Study of the endocytotic machinery via modification of viral-based nanoparticles</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Pharmaceutics

ISSN

0378-5173

e-ISSN

1873-3476

Volume of the periodical

611

Issue of the periodical within the volume

January

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

121308

UT code for WoS article

000779306000004

EID of the result in the Scopus database

2-s2.0-85119848021