Engineering the Ligand Specificity of the Human Galectin-1 by Incorporation of Tryptophan Analogues
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00556399" target="_blank" >RIV/61388963:_____/22:00556399 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/cbic.202100593" target="_blank" >https://doi.org/10.1002/cbic.202100593</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.202100593" target="_blank" >10.1002/cbic.202100593</a>
Alternative languages
Result language
angličtina
Original language name
Engineering the Ligand Specificity of the Human Galectin-1 by Incorporation of Tryptophan Analogues
Original language description
Galectin-1 is a beta-galactoside-binding lectin with manifold biological functions. A single tryptophan residue (W68) in its carbohydrate binding site plays a major role in ligand binding and is highly conserved among galectins. To fine tune galectin-1 specificity, we introduced several non-canonical tryptophan analogues at this position of human galectin-1 and analyzed the resulting variants using glycan microarrays. Two variants containing 7-azatryptophan and 7-fluorotryptophan showed a reduced affinity for 3'-sulfated oligosaccharides. Their interaction with different ligands was further analyzed by fluorescence polarization competition assay. Using molecular modeling we provide structural clues that the change in affinities comes from modulated interactions and solvation patterns. Thus, we show that the introduction of subtle atomic mutations in the ligand binding site of galectin-1 is an attractive approach for fine-tuning its interactions with different ligands.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chembiochem
ISSN
1439-4227
e-ISSN
1439-7633
Volume of the periodical
23
Issue of the periodical within the volume
5
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
e202100593
UT code for WoS article
000744320500001
EID of the result in the Scopus database
2-s2.0-85122926859