The rate of formation and stability of abasic site interstrand crosslinks in the DNA duplex

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00556771" target="_blank" >RIV/61388963:_____/22:00556771 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.dnarep.2022.103300" target="_blank" >https://doi.org/10.1016/j.dnarep.2022.103300</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dnarep.2022.103300" target="_blank" >10.1016/j.dnarep.2022.103300</a>

Result language
angličtina
Original language name
The rate of formation and stability of abasic site interstrand crosslinks in the DNA duplex
Original language description
DNA interstrand crosslinks (ICLs) strands pose an impenetrable barrier for DNA replication. Different ICLs are known to recruit distinct DNA repair pathways. NEIL3 glycosylase has been known to remove an abasic (Ap) site derived DNA crosslink (Ap-ICL). An Ap-ICL forms spontaneously from the Ap site with an adjacent adenine in the opposite strand. Lack of genetic models and a poor understanding of the fate of these lesions leads to many questions about the occurrence and the toxicity of Ap-ICL in cells. Here, we investigate the circumstances of Ap-ICL formation. With an array of different oligos, we have investigated the rates of formation, the yields, and the stability of Ap-ICL. Our findings point out how different bases in the vicinity of the Ap site change crosslink formation in vitro. We reveal that AT-rich rather than GC-rich regions in the surrounding Ap site lead to higher rates of Ap-ICL formation. Overall, our data reveal that Ap-ICL can be formed in virtually any DNA sequence context surrounding a hot spot of a 5′-Ap-dT pair, albeit with significantly different rates and yields. Based on Ap-ICL formation in vitro, we attempt to predict the number of Ap-ICLs in the cell.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GJ17-21649Y" target="_blank" >GJ17-21649Y: Dissecting the mechanisms and the role of FANCD2 monoubiquitylation in DNA crosslink repair</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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