Comprehensive analysis of glycerolipid dynamics during tobacco pollen germination and pollen tube growth

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00565625" target="_blank" >RIV/61388963:_____/22:00565625 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/22:00565625

Result on the web

<a href="https://doi.org/10.3389/fpls.2022.1028311" target="_blank" >https://doi.org/10.3389/fpls.2022.1028311</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2022.1028311" target="_blank" >10.3389/fpls.2022.1028311</a>

Result language

angličtina

Original language name

Comprehensive analysis of glycerolipid dynamics during tobacco pollen germination and pollen tube growth

Original language description

Pollen germination and subsequent pollen tube elongation are essential for successful land plant reproduction. These processes are achieved through well-documented activation of membrane trafficking and cell metabolism. Despite this, our knowledge of the dynamics of cellular phospholipids remains scarce. Here we present the turnover of the glycerolipid composition during the establishment of cell polarity and elongation processes in tobacco pollen and show the lipid composition of pollen plasma membrane-enriched fraction for the first time. To achieve this, we have combined several techniques, such as lipidomics, plasma membrane isolation, and live-cell microscopy, and performed a study with different time points during the pollen germination and pollen tube growth. Our results showed that tobacco pollen tubes undergo substantial changes in their whole-cell lipid composition during the pollen germination and growth, finding differences in most of the glycerolipids analyzed. Notably, while lysophospholipid levels decrease during germination and growth, phosphatidic acid increases significantly at cell polarity establishment and continues with similar abundance in cell elongation. We corroborated these findings by measuring several phospholipase activities in situ. We also observed that lysophospholipids and phosphatidic acid are more abundant in the plasma membrane-enriched fraction than that in the whole cell. Our results support the important role for the phosphatidic acid in the establishment and maintenance of cellular polarity in tobacco pollen tubes and indicate that plasma membrane lysophospholipids may be involved in pollen germination.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Volume of the periodical

13

Issue of the periodical within the volume

NOV 8

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

1028311

UT code for WoS article

000890615500001

EID of the result in the Scopus database

2-s2.0-85142217167