Cell-Interactive Gelatin-Based 19F MRI Tracers: An In Vitro Proof-of-Concept Study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00580553" target="_blank" >RIV/61388963:_____/24:00580553 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11320/24:10473753 RIV/00216208:11110/24:10473753

Result on the web

<a href="https://doi.org/10.1021/acs.chemmater.3c01574" target="_blank" >https://doi.org/10.1021/acs.chemmater.3c01574</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.chemmater.3c01574" target="_blank" >10.1021/acs.chemmater.3c01574</a>

Result language

angličtina

Original language name

Cell-Interactive Gelatin-Based 19F MRI Tracers: An In Vitro Proof-of-Concept Study

Original language description

Cross-linked gelatin-based hydrogels are highly promising cell-interactive, biocompatible, and biodegradable materials serving tissue engineering. Moreover, gelatins with covalently bound methacrylamide (gel-MA) and 2-aminoethyl methacrylate moieties (gel-AEMA) can be cross-linked through ultraviolet (UV) irradiation, which allows light-based three-dimensional (3D)-printing of such hydrogels. Furthermore, the physicochemical and biological properties of these hydrogels can be broadly tuned by incorporating various comonomers into the polymer chains, which makes these hydrogels a widely applicable platform in tissue engineering and reconstructive surgery. However, monitoring the degradation rate of hydrogel-based implants in vivo is challenging, thereby prohibiting their broad clinical transition and further research. Therefore, herein, we describe the synthesis of 3D-printable gelatin-based hydrogels with N-(2,2-difluoroethyl)acrylamide (DFEA), detectable with the chemical shift of −123 ppm, which enables us to monitor these implants in vivo with 19F magnetic resonance imaging (MRI) and assess their degradation kinetics. Next, we describe the physicochemical and biological properties of these hydrogels. Adding DFEA monomers into the reaction mixture accelerates their cross-linking kinetics. Moreover, increasing the DFEA content within the hydrogels increases their swelling ratio and 19F MRI signal. All hydrogels were detectable at small quantities (<16 mg) using 19F MRI. Moreover, our hydrogels supported the cell proliferation of adipose tissue-derived stem cells (ASCs) and had tunable biodegradation rates. Finally, we present a strategy for increasing the DFEA content without affecting the mechanical properties. Our results may be implemented in the future development of hydrogel implants, whose fate and biodegradation rate can be monitored via 19F MRI.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry of Materials

ISSN

0897-4756

e-ISSN

1520-5002

Volume of the periodical

36

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

183-196

UT code for WoS article

001139461500001

EID of the result in the Scopus database

2-s2.0-85179039557