19F MRI In Vivo Monitoring of Gelatin-Based Hydrogels: 3D Scaffolds with Tunable Biodegradation toward Regenerative Medicine

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00585751" target="_blank" >RIV/61388963:_____/24:00585751 - isvavai.cz</a>

Alternative codes found

RIV/61389013:_____/24:00585751 RIV/00216208:11110/24:10482618 RIV/00064165:_____/24:10482618

Result on the web

<a href="https://doi.org/10.1021/acs.chemmater.3c03321" target="_blank" >https://doi.org/10.1021/acs.chemmater.3c03321</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.chemmater.3c03321" target="_blank" >10.1021/acs.chemmater.3c03321</a>

Result language

angličtina

Original language name

19F MRI In Vivo Monitoring of Gelatin-Based Hydrogels: 3D Scaffolds with Tunable Biodegradation toward Regenerative Medicine

Original language description

Gelatin-based hydrogels emerged as promising biodegradable cell-compatible 3D-printable materials with tunable mechanical properties that serve tissue engineering and applications in regenerative medicine. Nevertheless, these materials are very challenging to monitor in vivo, which has hampered the further development of these materials and their translation into clinical practice. To overcome this limitation, we designed a cross-linked 3D-printable gelatin-based hydrogel endowed with poly[N-(2,2-difluoroethyl)acrylamide] (PDFEA). Such PDFEA-containing hydrogels can be monitored in vivo through fluorine-19 magnetic resonance imaging (19F MRI), which enables to monitor such implants in vivo and to assess their in vivo biodegradation kinetics. Herein, we prepared three different PDFEA-containing hydrogels with varying cross-linking degrees and studied their physicochemical properties (storage modulus, Young’s modulus, swelling ratio, in vitro degradation rate). Next, we administered these samples subcutaneously into mice and exploited 19F MRI to detect the biodegradation kinetics over 370 days. Hydrogels with a high cross-linking degree did not extensively degrade in vitro nor in vivo within the evaluated time frame. In contrast, hydrogels characterized by a low degree of cross-linking extensively degraded in vitro as well as in vivo (half-life of 228 ± 21 days). We demonstrated that endowing hydrogels with PDFEA enables monitoring of these hydrogels in vivo. Our results may become a benchmark in forthcoming studies of biodegradable hydrogels and the development of 19F MRI detectable gelatin-based hydrogels, paving the way toward their entry in clinical practice.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry of Materials

ISSN

0897-4756

e-ISSN

1520-5002

Volume of the periodical

36

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

4417-4425

UT code for WoS article

001226362400001

EID of the result in the Scopus database

2-s2.0-85191156450