Predicting the Structural Effects of CUG Codon Translation on Uncharacterized Proteins in Candida albicans

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00639712" target="_blank" >RIV/61388963:_____/25:00639712 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/25:10503750

Result on the web

<a href="https://doi.org/10.3390/jof11090638" target="_blank" >https://doi.org/10.3390/jof11090638</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/jof11090638" target="_blank" >10.3390/jof11090638</a>

Result language

angličtina

Original language name

Predicting the Structural Effects of CUG Codon Translation on Uncharacterized Proteins in Candida albicans

Original language description

In the standard genetic code, the CUG triplet is translated as leucine. The pathogenic yeast Candida albicans and other CTG-clade yeasts contain tRNACAG, which is recognized by both leucine- and serine-tRNA synthetases. The CUG codon in these yeasts is translated most often as serine, and only in 3-5% of cases as leucine. Therefore, CTG Candida species have unstable proteomes. The effect of serine-leucine exchange on the structure and function of proteins has only been experimentally examined for a few cases. In C. albicans, CUG codons occur even in genes deemed to be essential. This means that serine-leucine ambiguity either does not affect the structure and function of the respective proteins, or that the presence of these amino acids at specific positions is associated with meaningful alteration of the proteins' function. This study employed AlphaFold2 to evaluate the potential effects of serine-to-leucine exchange in 12 proteins encoded by essential genes lacking orthologs in other yeasts and human genomes. The low homology with known proteins allowed us to make only low-confidence predictions. The analyzed proteins could be grouped into subsets based on the structural outcomes. Structural changes were observed only in four proteins. The remaining eight proteins showed no significant differences between serine and leucine variants.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Fungi

ISSN

2309-608X

e-ISSN

2309-608X

Volume of the periodical

11

Issue of the periodical within the volume

9

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

638

UT code for WoS article

001580167100001

EID of the result in the Scopus database

2-s2.0-105017471447