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The relationship between the modular structure of eucaryotic-like serine/threonine protein kinase StkP of Streptococcus pneumoniae and its function

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F04%3A00100409" target="_blank" >RIV/61388971:_____/04:00100409 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The relationship between the modular structure of eucaryotic-like serine/threonine protein kinase StkP of Streptococcus pneumoniae and its function

  • Original language description

    In order to determine a cellular localization of protein kinase StkP we prepared its epitope-labelled form expressed in S. pneumoniae. The full-length form of StkP was detected with monoclonal antibody in the membrane fraction. In vitro autophosphorylation assays confirmed this finding and phosphorylated product of corresponding mass was detected in the membranes. These results clearly showed that StkP is a membrane-associated protein.

  • Czech name

    Vztah mezi modulární strukturou serin/threonin proteinkinasy eukaryontního typu StkP z Streptococcus pneumoniae a její funkcí

  • Czech description

    Byla určena buněčná lokalizace proteinkinasy StkP pomocí integrace do genomu S. pneumoniae jejího genu označeného epitopem. Nativní forma StkP byla detekována monoklonální protilátkou v membránové frakci. Tento výsledek byl ověřen in vitro autofosforylací a fosforylovaný produkt odpovídající velikosti byl detekován v membránách.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    European Congress of Clinical Microbiology and Infectious Diseases /14./

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    522

  • Publisher name

  • Place of publication

    Praha

  • Event location

    Praha

  • Event date

    May 1, 2004

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article