A eukaryotic-type serine/threonine protein kinase StkP of Streptococcus pneumoniae acts as a dimer in vivo

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F07%3A00085739" target="_blank" >RIV/61388971:_____/07:00085739 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

A eukaryotic-type serine/threonine protein kinase StkP of Streptococcus pneumoniae acts as a dimer in vivo

Original language description

Streptococcus pneumoniae carries a single Ser/Thr protein kinase gene stkP in its genome. Biochemical studies performed with recombinant StkP have revealed that this protein is a functional membrane-linked eukaryotic-type Ser/Thr protein kinase. Here, wedemonstrate that the deletion of its extracellular domain negatively affects the stability of a core kinase domain. In contrast, the membrane anchored kinase domain and the full-length form of StkP were stable and capable of autophosphorylation. Furthermore, evidence is presented that StkP forms dimers through its transmembrane and extracellular domains

Czech name

Serin-threoninová proteinkinasa StkP eukaryotického typu Streptococcus pneumoniae působí v podmínkách in vivo jako dimer

Czech description

Streptococcus pneumoniae má ve svém genomu jediný gen kódujícíc serin-threoninovou proteinkinasu StkP. Biochemické studie provedené s rekombinantní StkP prokázaly, že tento protein je funkční membránovou serin-threoninovou proteinkinasou eukaryotického typu. Prokázali jsme, že delece její extracelulární domény negativně ovlivňuje stabilitu domény kinázové. Naopak, kinázová doména zakotvená v membráně a celá StkP jsou stabilní a schopné autofosforylace. Dále, StkP vytváří dimery skrze transmembránovou aextracelulární domény

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

—

Project

<a href="/en/project/GA204%2F02%2F1423" target="_blank" >GA204/02/1423: Relationship between activity of protein Ser/Thr kinases and inter- and intracellular signalling in differentiating and pathogenical bacterial species</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemical and Biophysical Research Communications

ISSN

0006-291X

e-ISSN

—

Volume of the periodical

355

Issue of the periodical within the volume

-

Country of publishing house

US - UNITED STATES

Number of pages

5

Pages from-to

526-530

UT code for WoS article

—

EID of the result in the Scopus database

—