Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00040292" target="_blank" >RIV/61388971:_____/06:00040292 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae

Original language description

The hexose-proton symporter HUP1 shows a spotty distribution in the plasma membrane of the green alga Chlorella kessleri. Chlorella cannot be transformed so far. To study the membrane localization of the HUP1 protein in detail, the symporter was fused togreen fluorescent protein (GFP) and heterologously expressed in Saccharomyces cerevisiae and Schizosaccharomyces pombe. In these organisms, the HUP1 protein has previously been shown to be fully active. The GFP fusion protein was exclusively targeted tothe plasma membranes of both types of fungal cells. In S. cerevisiae, it was distributed nonhomogenously and concentrated in spots resembling the patchy appearance observed previously for endogenous H(+) symporters. It is documented that the Chlorella protein colocalizes with yeast proteins that are concentrated in 300-nm raft-based membrane compartments

Czech name

Kompartmentace rostlinného transporteru založená na lipidech

Czech description

Hexose-proton symporter HUP1 je v plasmatické membráně zelené řasy Chlorella kessleri distribuován v pačích.. Protože Chlorella nemůže být transformována, localizace HUP1 proteinu byla sledováno po připojeni zeleného fluorescenčního proteinu GFP a heterologní expresi v Saccharomyces cerevisiae beno Schizosaccharomyces pombe. HUP1-GFP fusní protein byl targetován do plasmatické membrany těchto organismů.V S. cerevisiaebyl distribuován nehomogenně a koncentrován v pačích podobně jako bylo prokázáno pro kvasničné endogenní proteiny. S. cerevisiae mutanty , které neobsahují typické raftové lipidy ergosterol l sphingolipidy vykazují homogenní distribuci HUP1-GFP. V mutantu erg6 domains mohou být in vivo barveny filipinem

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/LC545" target="_blank" >LC545: Functional Organisation of the Cell</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Eukaryotic Cell

ISSN

1535-9778

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

945-953

UT code for WoS article

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EID of the result in the Scopus database

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