An Increase in Antimycobacterial Th1-Cell Responses by Prime-Boost Protocols of Immunization Does Not Enhance Protection against Tuberculosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00040340" target="_blank" >RIV/61388971:_____/06:00040340 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
An Increase in Antimycobacterial Th1-Cell Responses by Prime-Boost Protocols of Immunization Does Not Enhance Protection against Tuberculosis
Original language description
Bordetella pertussis adenylate cyclase (CyaA) toxoid is a powerful nonreplicative immunization vector targeting dendritic cells, which has already been used successfully in prophylactic and therapeutic vaccination in various preclinical animal models. Here, we investigated the potential of CyaA, harboring strong mycobacterial immunogens, i.e., the immunodominant regions of antigen 85A or the complete sequence of the 6-kDa early secreted antigenic target (ESAT-6) protein, to induce antimycobacterial immunity. By generating T-cell hybridomas or by using T cells from mice infected with mycobacteria, we first demonstrated that the in vitro delivery of 85A or ESAT-6 to antigen-presenting cells by CyaA leads to processing and presentation, by major histocompatibility complex class II molecules, of the same epitopes as those displayed upon mycobacterial infection
Czech name
Nárůst antimykobakteriální TH1-buněčné odpovědi po Prime-Boost imunizaci nezvyšuje ochrannou imunitu proti tuberkulóze
Czech description
Práce ukazuje, že nárůst antimykobakteriální TH1-buněčné odpovědi po Prime-Boost imunizaci nezvyšuje ochrannou imunitu proti tuberkulóze. Byly identifikovány imunodominantní epitopy antigenu ESAT-6, připraveny specifické hybridomy a ukázáno, že imunizacepomocí toxoidů CyaA jež nesou antigen ESAT-6 vede ke značnému nárůstu imunitní odpovědi specifických T lymfocytů Th1 a produkci interferonu gama. To ale nezaručuje ochrannou imunitu proti tuberkulóze, což je nové poznání odporující ustáleným pradigmatůmv oboru
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/IBS5020311" target="_blank" >IBS5020311: Vaccines for induction of mucosal and systemic immunity</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Infection and Immunity
ISSN
0019-9567
e-ISSN
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Volume of the periodical
74
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
2128-2137
UT code for WoS article
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EID of the result in the Scopus database
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