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HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00043244" target="_blank" >RIV/61388971:_____/06:00043244 - isvavai.cz</a>

  • Alternative codes found

    RIV/00027162:_____/06:#0000098

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets

  • Original language description

    Escherichia coli A0 34/86 (O83:K24:H31) has been successfully used for prophylactic and therapeutic intestinal colonization of premature and newborn infants, with the aim of preventing nosocomial infections. Although E. coli A0 34/86 was described as a nonpathogenic commensal, partial sequencing revealed that its genome harbours gene clusters highly homologous to virulence determinants of different types of E. coli, including closely linked genes of the a-haemolysin operon (hlyCABD) and for the cytotoxic necrotizing factor (cnf1). A haemolysin-deficient mutant (hlyA) of E. coli A0 34/86 was generated and its colonization capacity was determined. The results show that a single dose of the A0 34/86 wild-type or hlyA strains resulted in efficient intestinal colonization of newborn conventional piglets, and that this was still considerable after several weeks

  • Czech name

    Vyřazení HlyA vede k bezpečnější variantě kmene Escherichia coli A0 34/86 s nezměněnými kolonizačními schopnostmi

  • Czech description

    Kmen Escherichia coli A0 34/86 (O83:K24:H31) je úspěšně používán pro preventivní kolonizaci novorozenců. V této práci ukazujeme, že jeho bezpečnost lze ještě vylepšit odstraněním genu pro hemolyzín, což neovlivní kolonizační schopnosti kmene v prasečím experimentálním modelu

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA523%2F03%2F0186" target="_blank" >GA523/03/0186: Recombinant E. coli O83 as a live vector for mucosal vaccines</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    FEMS Immunology and Medical Microbiology

  • ISSN

    0928-8244

  • e-ISSN

  • Volume of the periodical

    48

  • Issue of the periodical within the volume

    -

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    12

  • Pages from-to

    257-266

  • UT code for WoS article

  • EID of the result in the Scopus database