HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00043244" target="_blank" >RIV/61388971:_____/06:00043244 - isvavai.cz</a>
Alternative codes found
RIV/00027162:_____/06:#0000098
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets
Original language description
Escherichia coli A0 34/86 (O83:K24:H31) has been successfully used for prophylactic and therapeutic intestinal colonization of premature and newborn infants, with the aim of preventing nosocomial infections. Although E. coli A0 34/86 was described as a nonpathogenic commensal, partial sequencing revealed that its genome harbours gene clusters highly homologous to virulence determinants of different types of E. coli, including closely linked genes of the a-haemolysin operon (hlyCABD) and for the cytotoxic necrotizing factor (cnf1). A haemolysin-deficient mutant (hlyA) of E. coli A0 34/86 was generated and its colonization capacity was determined. The results show that a single dose of the A0 34/86 wild-type or hlyA strains resulted in efficient intestinal colonization of newborn conventional piglets, and that this was still considerable after several weeks
Czech name
Vyřazení HlyA vede k bezpečnější variantě kmene Escherichia coli A0 34/86 s nezměněnými kolonizačními schopnostmi
Czech description
Kmen Escherichia coli A0 34/86 (O83:K24:H31) je úspěšně používán pro preventivní kolonizaci novorozenců. V této práci ukazujeme, že jeho bezpečnost lze ještě vylepšit odstraněním genu pro hemolyzín, což neovlivní kolonizační schopnosti kmene v prasečím experimentálním modelu
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA523%2F03%2F0186" target="_blank" >GA523/03/0186: Recombinant E. coli O83 as a live vector for mucosal vaccines</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEMS Immunology and Medical Microbiology
ISSN
0928-8244
e-ISSN
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Volume of the periodical
48
Issue of the periodical within the volume
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Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
12
Pages from-to
257-266
UT code for WoS article
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EID of the result in the Scopus database
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