Specific regions of genome plasticity and genetic diversity of the commensal Escherichia coli A0 34/86
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00044187" target="_blank" >RIV/61388971:_____/06:00044187 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Specific regions of genome plasticity and genetic diversity of the commensal Escherichia coli A0 34/86
Original language description
Escherichia coli A0 34/86 (O83.K24.H31) is a commensal strain that has been used for prophylactic and therapeutic colonization of the intestine of newborn infants. To identify traits specific for E. coli A0 34/86, we used a minimal tiling set of 148 BACclones of A0 34/86 genomic DNA, to construct restriction-digested BAC arrays. Hybridization with genomic DNA from four E. coli strains (CFT073, O157.H7, K12 and Nissle 1917) allowed selection of two BAC clones that were sequenced to identify A0 34/86-specific regions. Genes for the yersiniabactin siderophore system, several proteins homologous to Salmonella enterica serovar Typhimurium vitamin B12 synthesis proteins, as well as genes necessary for the degradation of propanediol, the pix fimbriae determinant and genes coding for a putative phosphoglycerate transport system present also on pathogenicity island V of E. coli strain 536 were all identified in E. coli A0 34/86
Czech name
Specifické oblasti genomové plasticity a genetická diverzita komenzálního kmene Escherichia coli A0 34/86
Czech description
Escherichia coli A0 34/86 (O83.K24.H31) je komenzální kmen používaný k profylaktické a terapeutické kolonizaci novorozenců. K získání specifických vlastností E. coli A0 34/86 jsme zkonstruovali BAC arrays knihovnu za použití 148 BAC klonů A0 34/86 genomové DNA. Hybridizace BAC arrays s genomovou DNA čtyř kmenů E. coli (CFT073, O157.H7, K12 a Nissle 1917) umožnila selekci dvou BAC klonů, které byly sekvenovány. E. coli A0 34/86 obsahuje geny pro yersiniabactin siderophore systém, geny homologní ke genůmpro syntézu vitamínu B12 u Salmonella enterica sérovar Typhimurium, geny nezbytné pro degradaci propanediolu, geny pro pix fimbrie a geny k?dující transport fosfoglycerátu. Tato komparativní analýza podtrhuje také důležitou genomovou heterogenitu mezi kmeny E. coli
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Medical Microbiology
ISSN
1438-4221
e-ISSN
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Volume of the periodical
296
Issue of the periodical within the volume
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Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
541-546
UT code for WoS article
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EID of the result in the Scopus database
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