LC MALDI-TOF MS/MS and LC ESI FTMS analyses of HLA-B27 associated peptides isolated from peripheral blood cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F08%3A00312354" target="_blank" >RIV/61388971:_____/08:00312354 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/08:00312354 RIV/00023001:_____/08:00001659

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

LC MALDI-TOF MS/MS and LC ESI FTMS analyses of HLA-B27 associated peptides isolated from peripheral blood cells

Original language description

Peptides eluted from peripheral blood cells of HLA-B 2705 healthy donor were analyzed by LC MALDI MS/MS and LC ESI FTMS techniques. The sequences of 92 peptide ligands identified from one healthy blood donor by LC MALDI-TOF MS/MS were compared with thosepreviously published. It was found that 18 sequences confirmed within 1 ppm mass error by LC ESI FTMS were already described and 3 of them matched with those previously reported from HLA-B 2705 splenocytes. Another 38 sequences validated within the samemass error were not found in SYFPEITHI database and are identified here for the first time. Finally, 36 sequences (5 sequences already published in SYFPEITHI database) were evaluated by LC MALDI-TOF MS/MS but no matches in the list of monoisotopic masses obtained from LC ESI FTMS were found

Czech name

LC MALDI-TOF MS/MS a LC ESI FTMS analýzy HLA-B27 asociovaných peptidů izolovaných z buněk periferní krve

Czech description

Peptidy eluované z krevních buněk HLA-B 2705 zdravých dárců byly analyzovány metodami LC MALDI MS/MS a LC ESI FTMS. Bylo identifikováno 92 peptidů z 1 zdravého dárce metodou LC MALDI-TOF MS/MS. 18 peptidových sekvencí získaných LC ESI FTMS (1 ppm) bylo známých a 3 z nich byly nalezeny u HLA-B 2705 splenocytů. Dalších 38 sekvencí nebylo nalezeno v SYFPEITHI databázi a jsou zde prezentovány poprvé. Nakonec, 36 sekvencí (5 peptidů publikovaných v SYFPEITHI databázi) získaných pomocí LC MALDI-TOF MS/MS nebyly detekovány metodou LC ESI FTMS

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/1A8631" target="_blank" >1A8631: Sequence of peptides eluted from HLA-B27 molecules in healthy and diseased individuals</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Immunology Letters

ISSN

0165-2478

e-ISSN

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Volume of the periodical

116

Issue of the periodical within the volume

-

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

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UT code for WoS article

000262515800018

EID of the result in the Scopus database

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