Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F11%3A00373580" target="_blank" >RIV/61388971:_____/11:00373580 - isvavai.cz</a>
Alternative codes found
RIV/67985831:_____/11:00373580
Result on the web
<a href="http://dx.doi.org/10.1111/j.1574-6968.2011.02243.x" target="_blank" >http://dx.doi.org/10.1111/j.1574-6968.2011.02243.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1574-6968.2011.02243.x" target="_blank" >10.1111/j.1574-6968.2011.02243.x</a>
Alternative languages
Result language
angličtina
Original language name
Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers
Original language description
A reliable method of detection in roots and soil is thus needed for assessment of geographic distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage wasthen used to distinguish the two species. The occurrence of T. aestivum in soil and ectomycorrhizae collected in its native habitat has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP504%2F10%2F0382" target="_blank" >GAP504/10/0382: Tuber aestivum Vitt.: a study of distribution, biology and cultivation with the perspective of future practical exploitation</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEMS Microbiology Letters
ISSN
0378-1097
e-ISSN
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Volume of the periodical
318
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
84-91
UT code for WoS article
000289158900012
EID of the result in the Scopus database
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