Changes in activity of metabolic and regulatory pathways during germination of S-coelicolor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00506585" target="_blank" >RIV/61388971:_____/14:00506585 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/14:00439959 RIV/00216208:11110/14:10293640 RIV/44555601:13440/14:43886277

Result on the web

<a href="https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-1173" target="_blank" >https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-1173</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1471-2164-15-1173" target="_blank" >10.1186/1471-2164-15-1173</a>

Result language

angličtina

Original language name

Changes in activity of metabolic and regulatory pathways during germination of S-coelicolor

Original language description

Background: Bacterial spore germination is a developmental process during which all required metabolic pathways are restored to transfer cells from their dormant state into vegetative growth. Streptomyces are soil dwelling filamentous bacteria with complex life cycle, studied mostly for they ability to synthesize secondary metabolites including antibiotics. nnResults: Here, we present a systematic approach that analyzes gene expression data obtained from 13 time points taken over 5.5 h of Streptomyces germination. Genes whose expression was significantly enhanced/diminished during the time-course were identified, and classified to metabolic and regulatory pathways. The classification into metabolic pathways revealed timing of the activation of specific pathways during the course of germination. The analysis also identified remarkable changes in the expression of specific sigma factors over the course of germination. Based on our knowledge of the targets of these factors, we speculate on their possible roles during germination. Among the factors whose expression was enhanced during the initial part of germination, SigE is though to manage cell wall reconstruction, SigR controls protein re-aggregation, and others (SigH, SigB, SigI, SigJ) control osmotic and oxidative stress responses. nnConclusions: From the results, we conclude that most of the metabolic pathway mRNAs required for the initial phases of germination were synthesized during the sporulation process and stably conserved in the spore. After rehydration in growth medium, the stored mRNAs are being degraded and resynthesized during first hour. From the analysis of sigma factors we conclude that conditions favoring germination evoke stress-like cell responses.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Genomics

ISSN

1471-2164

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

DEC 23

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

1173

UT code for WoS article

000347731600001

EID of the result in the Scopus database

2-s2.0-84928235774