Dunaliella salina (Chlorophyceae) affects the quality of NaCl crystals
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00506706" target="_blank" >RIV/61388971:_____/14:00506706 - isvavai.cz</a>
Result on the web
<a href="https://bioone.org/journals/cryptogamie-algologie/volume-35/issue-3/crya.v35.iss3.2014.285/Dunaliella-salina-Chlorophyceae-Affects-the-Quality-of-NaCl-Crystals/10.7872/crya.v35.iss3.2014.285.short" target="_blank" >https://bioone.org/journals/cryptogamie-algologie/volume-35/issue-3/crya.v35.iss3.2014.285/Dunaliella-salina-Chlorophyceae-Affects-the-Quality-of-NaCl-Crystals/10.7872/crya.v35.iss3.2014.285.short</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7872/crya.v35.iss3.2014.285" target="_blank" >10.7872/crya.v35.iss3.2014.285</a>
Alternative languages
Result language
angličtina
Original language name
Dunaliella salina (Chlorophyceae) affects the quality of NaCl crystals
Original language description
The green alga Dunaliella salina is a common inhabitant of crystallizers in solar saltworks. Although its presence has been associated with low salt quality, little is known about the mechanism through which Dunaliella causes such effect. It has been suggested that the release of organic matter from healthy D. salina cells interferes with NaCl crystallization. With the intention to cast some light on this matter, we grew D. salina cells at different [NaCl] (0.3, 1.0, 2.0 and 3.0 M), in the presence of either NO3- or NH4+, in conditions that, although strongly hyperaline, were mild enough to minimize cell lysis and substantial spill over of cytosolic sap. The two N treatments were done because the N-source was shown to influence organic release from D. salina. We then attempted to link this physiological information to the information to the quality of NaCl crystals. In order to reduce the impact of inter-crystal inclusion and focus on the structure of the crystalline reticulum, the volume of water used for the production of crystals was as small as possible. Photosynthesis (as chlorophyll variable fluorescence associated with photosystem II), cell organic (as determined by Fourier Transform Infrared spectroscopy, FTIR) and elemental (measured by total reflection X ray fluorescence spectrometry, TXRF) composition were analyzed at the beginning and in the middle of the exponential growth phase, and in the stationary phase. The quality of the crystals obtained from sterile media and from D. salina growth media was studied by optical microscopy (to determine the shape of the crystals) and by X-ray diffractometry. nnOur results demonstrate that the presence of D. salina cells is directly connected to alterations of NaCl crystalline structure (specifically of crystallite size) and with the quantity and quality of contaminants in the salt.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cryptogamie, Algologie
ISSN
0181-1568
e-ISSN
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Volume of the periodical
35
Issue of the periodical within the volume
3
Country of publishing house
FR - FRANCE
Number of pages
6
Pages from-to
285-302
UT code for WoS article
000343029700006
EID of the result in the Scopus database
2-s2.0-84906857203