Physiological roles of sigma factor SigD in Corynebacterium glutamicum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00477066" target="_blank" >RIV/61388971:_____/17:00477066 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1186/s12866-017-1067-6" target="_blank" >http://dx.doi.org/10.1186/s12866-017-1067-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12866-017-1067-6" target="_blank" >10.1186/s12866-017-1067-6</a>
Alternative languages
Result language
angličtina
Original language name
Physiological roles of sigma factor SigD in Corynebacterium glutamicum
Original language description
Background: Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail, however, the role of SigD in transcriptional regulation in C. glutamicum has been mostly unknown. nResults: In this work, pleiotropic effects of sigD overexpression at the level of phenotype, transcripts, proteins and metabolites were investigated. Overexpression of sigD decreased the growth rate of C. glutamicum cultures, and induced several physiological effects such as reduced culture foaming, turbid supernatant and cell aggregation. Upon overexpression of sigD, the level of Cmt1 (corynomycolyl transferase) in the supernatant was notably enhanced, and carbohydrate-containing compounds were excreted to the supernatant. The real-time PCR analysis revealed that sigD overexpression increased the expression of genes related to corynomycolic acid synthesis (fadD2, pks), genes encoding corynomycolyl transferases (cop1, cmt1, cmt2, cmt3), L, D-transpeptidase (lppS), a subunit of the major cell wall channel (porH), and the envelope lipid regulation factor (elrF). Furthermore, overexpression of sigD resulted in trehalose dicorynomycolate accumulation in the cell envelope. nConclusions: This study demonstrated that SigD regulates the synthesis of corynomycolate and related compounds, and expanded the knowledge of regulatory functions of sigma factors in C. glutamicum.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA17-06991S" target="_blank" >GA17-06991S: Construction of a model of regulatory network controlled by sigma factors of RNA polymerase in Corynebacterium glutamicum</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC Microbiology
ISSN
1471-2180
e-ISSN
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Volume of the periodical
17
Issue of the periodical within the volume
158
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
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UT code for WoS article
000405422000002
EID of the result in the Scopus database
2-s2.0-85023166489