The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00482204" target="_blank" >RIV/61388971:_____/17:00482204 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/17:10366566
Result on the web
<a href="http://dx.doi.org/10.1038/s41467-017-01542-z" target="_blank" >http://dx.doi.org/10.1038/s41467-017-01542-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-017-01542-z" target="_blank" >10.1038/s41467-017-01542-z</a>
Alternative languages
Result language
angličtina
Original language name
The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding
Original language description
Vaccinia virus (VACV), the prototype member of the Poxviridae, replicates in the cytoplasm of an infected cell. The catalytic subunit of the DNA polymerase E9 binds the heterodimeric processivity factor A20/D4 to form the functional polymerase holoenzyme. Here we present the crystal structure of full-length E9 at 2.7 angstrom resolution that permits identification of important poxvirus-specific structural insertions. One insertion in the palm domain interacts with C-terminal residues of A20 and thus serves as the processivity factor-binding site. This is in strong contrast to all other family B polymerases that bind their co-factors at the C terminus of the thumb domain. The VACV E9 structure also permits rationalization of polymerase inhibitor resistance mutations when compared with the closely related eukaryotic polymerase delta-DNA complex.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LQ1604" target="_blank" >LQ1604: BIOCEV: from Fundamental to Applied Research</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Communications
ISSN
2041-1723
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
NOV 13
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
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UT code for WoS article
000414915900017
EID of the result in the Scopus database
2-s2.0-85033776744