Switching transcription with bacterial RNA polymerase through photocaging, photorelease and phosphorylation reactions in the major groove of DNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00505476" target="_blank" >RIV/61388971:_____/19:00505476 - isvavai.cz</a>

Alternative codes found

RIV/61388963:_____/19:00505476 RIV/00216208:11310/19:10394788

Result on the web

<a href="https://pubs.rsc.org/en/content/articlelanding/2019/SC/C9SC00205G#!divAbstract" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2019/SC/C9SC00205G#!divAbstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c9sc00205g" target="_blank" >10.1039/c9sc00205g</a>

Result language

angličtina

Original language name

Switching transcription with bacterial RNA polymerase through photocaging, photorelease and phosphorylation reactions in the major groove of DNA

Original language description

We report proof of principle biomimetic switching of transcription in vitro through non-natural chemical reactions in the major groove of DNA templates. Photocaged DNA templates containing nitrobenzyl-protected 5-hydroxymethyluracil or -cytosine permitted no transcription with E. coli RNA polymerase (OFF state). Their irradiation with 400 nm light resulted in DNA templates containing hydroxymethylpyrimidines, which switched transcription ON with a higher yield (250-350%) compared to non-modified DNA. Phosphorylation of templates containing 5-hydroxymethyluracil (but not 5-hydroxymethylcytosine) then turned transcription OFF again. It is the first step towards artificial bioorthogonal chemical epigenetics.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Science

ISSN

2041-6520

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

14

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

3937-3942

UT code for WoS article

000467988600024

EID of the result in the Scopus database

2-s2.0-85064060087