Turning Off Transcription with Bacterial RNA Polymerase through CuAAC Click Reactions of DNA Containing 5-Ethynyluracil
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00491770" target="_blank" >RIV/61388963:_____/18:00491770 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/18:00491770 RIV/00216208:11310/18:10378073
Result on the web
<a href="http://dx.doi.org/10.1002/chem.201801757" target="_blank" >http://dx.doi.org/10.1002/chem.201801757</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/chem.201801757" target="_blank" >10.1002/chem.201801757</a>
Alternative languages
Result language
angličtina
Original language name
Turning Off Transcription with Bacterial RNA Polymerase through CuAAC Click Reactions of DNA Containing 5-Ethynyluracil
Original language description
Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction in the major groove of DNA containing 5-ethynyluracil (U-E) with azides was used for turning off sequence-specific protein-DNA interactions. The concept was first demonstrated on switching off cleavage of short modified DNA by restriction endonuclease BamHI-HF. Finally, DNA template containing U-E was used for in vitro transcription with E.coli RNA polymerase and the transcription was turned off by CuAAC with 3-azidopropane-1,2-diol or 3-azido-7-hydroxycoumarin.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA17-03419S" target="_blank" >GA17-03419S: Artificial epigenetics: Development of a eukaryotic bioorthogonal transcription system for gene expression manipulation in the cell.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemistry - A European Journal
ISSN
0947-6539
e-ISSN
—
Volume of the periodical
24
Issue of the periodical within the volume
33
Country of publishing house
DE - GERMANY
Number of pages
4
Pages from-to
8311-8314
UT code for WoS article
000435269000012
EID of the result in the Scopus database
2-s2.0-85048638047