Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00509053" target="_blank" >RIV/61388971:_____/19:00509053 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.14265" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.14265</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jcmm.14265" target="_blank" >10.1111/jcmm.14265</a>
Alternative languages
Result language
angličtina
Original language name
Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells
Original language description
We report results showing that the silencing of carbonic anhydrase I (siCA1) in prostatic (PC3) tumour cells has a significant impact on exosome formation. An increased diameter, concentration and diversity of the produced exosomes were noticed as a consequence of this knock-down. The protein composition of the exosomes' cargo was also altered. Liquid chromatography and mass spectrometry analyses identified 42 proteins significantly altered in PC3 siCA1 exosomes compared with controls. The affected proteins are mainly involved in metabolic processes, biogenesis, cell component organization and defense/immunity. Interestingly, almost all of them have been described as 'enhancers' of tumour development through the promotion of cell proliferation, migration and invasion. Thus, our results indicate that the reduced expression of the CA1 protein enhances the malignant potential of PC3 cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Cellular and Molecular Medicine
ISSN
1582-4934
e-ISSN
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Volume of the periodical
23
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
3641-3655
UT code for WoS article
000466841100055
EID of the result in the Scopus database
2-s2.0-85064815688