Immunoglobulin light chain kappa precedes Lambda rearrangement in swine but a majority of Lambda+ B cells are generated earlier

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00532773" target="_blank" >RIV/61388971:_____/20:00532773 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0145305X20301749" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0145305X20301749</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dci.2020.103751" target="_blank" >10.1016/j.dci.2020.103751</a>

Result language
angličtina
Original language name
Immunoglobulin light chain kappa precedes Lambda rearrangement in swine but a majority of Lambda+ B cells are generated earlier
Original language description
Developmental pathways for B cell lymphogenesis are sufficiently known only in mice and humans. However, both of these species rearrange immunoglobulin heavy chains (IgH) before light chains (IgL) while IgL precedes IgH rearrangement in swine. We demonstrate here that this reversed order of rearrangements have some concealed consequences: (1) we confirmed that although IgLκ rearrangement is initial, most IgLλ+ B cells are generated earlier and before IgH rearrangements, while most IgLκ+ B cells later and after IgH rearrangements, (2) the second IgLκ rearrangement can occur after IgLλ rearrangement, (3) early formed B cells bear only single in-frame IgH rearrangements, (4) many IgLκ+ B cells carry IgLλ rearrangements that can be productive and occurring on both alleles in one cell, and (5) although VpreB and λ5 genes are present in swine, they are preferentially expressed in non-B cells. In summary, our findings reveal that swine use an alternative B cell developmental pathway as compared to mice and humans.nn
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10613 - Zoology

Project
<a href="/en/project/GA19-01504S" target="_blank" >GA19-01504S: Different order of immunoglobulin light chain rearrangement in swine</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)