The order of immunoglobulin light chain κ and λ usage in primary and secondary lymphoid tissues of germ-free and conventional piglets

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00556044" target="_blank" >RIV/61388971:_____/22:00556044 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0145305X22000544?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0145305X22000544?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.dci.2022.104392" target="_blank" >10.1016/j.dci.2022.104392</a>

Result language

angličtina

Original language name

The order of immunoglobulin light chain κ and λ usage in primary and secondary lymphoid tissues of germ-free and conventional piglets

Original language description

In pigs (Sus scrofa), the initial immunoglobulin rearrangement of the κ light chain is replaced by λ before the heavy chains rearrange, and the light chains may rearrange even later. This study investigates whether these developmental differences are reflected in the usage of IGK and IGL genes. We found large differences between peripheral B cells and those developing in the bone marrow, and between B cells in germ-free piglets and conventional pigs. During early B cell development in the bone marrow, more 3′ V and 5′ J gene segments for both light chains are used. However, in the peripheral naive repertoire, more 5′ IGLV and 3′ IGLJ genes are used. A similar shift toward the use of more 5′ IGKV and 3′ IGKJ genes is observed later after antigen exposure in conventional pigs. The expression profile showed that most λ+ B cells are generated earlier, while κ+ B cells develop from late precursors that already contain the λ rearrangement. The initial λ rearrangement is retained in both λ+ and κ+ B lymphocytes, and multiple λ transcripts can be found in individual cells. The overall pool of the IGLV repertoire is therefore much larger and more diversified than for IGKV. The κ repertoire is further restricted to the preferential use of only two major IGKV genes, reflecting the limitation for only two consecutive rearrangements. Tracing of silenced λ transcripts in κ+ B cells further confirmed the unconventional mechanism of differential rearrangements in pigs. Our results underline the diversity of the immune system among mammals.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

10613 - Zoology

Project

<a href="/en/project/GA19-01504S" target="_blank" >GA19-01504S: Different order of immunoglobulin light chain rearrangement in swine</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Developmental and Comparative Immunology

ISSN

0145-305X

e-ISSN

1879-0089

Volume of the periodical

131

Issue of the periodical within the volume

June 2022

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

104392

UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85125278336