Consequences of the different order of immunoglobulin gene rearrangements in swine

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00550862" target="_blank" >RIV/61388971:_____/22:00550862 - isvavai.cz</a>
Result on the web
<a href="https://www-sciencedirect-com.d360prx.biomed.cas.cz/science/article/pii/S0145305X21002044" target="_blank" >https://www-sciencedirect-com.d360prx.biomed.cas.cz/science/article/pii/S0145305X21002044</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dci.2021.104196" target="_blank" >10.1016/j.dci.2021.104196</a>

Result language
angličtina
Original language name
Consequences of the different order of immunoglobulin gene rearrangements in swine
Original language description
Swine use a reverse order of immunoglobulin chain rearrangement compared to humans and mice, and this altered and modified order should have measurable consequences. Here we perform new and defining experiments with developing and mature B cells, characterizing the B cell populations that do not exist in other species. First, we have finally confirmed that light chains kappa and lambda are rearranged and expressed on the surface before any heavy chain rearrangements using western-blot. And second, we have analyzed a pool of mature B cells on the single-cell level to demonstrate that many kappa+ mature B cells carry lambda transcripts. According to these findings, we believe that there may be more groups of mammals, one of which uses a pre-BCR-driven developmental pathway for B cell generation (like mice and humans), the second group uses a pre-BCR-independent one (like swine), and some may be even intermediate.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10613 - Zoology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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