MicroRNA dilution during oocyte growth disables the microRNA pathway in mammalian oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00537847" target="_blank" >RIV/61388971:_____/20:00537847 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/20:00537847 RIV/67985904:_____/20:00537847

Result on the web

<a href="https://academic.oup.com/nar/article/48/14/8050/5866099" target="_blank" >https://academic.oup.com/nar/article/48/14/8050/5866099</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkaa543" target="_blank" >10.1093/nar/gkaa543</a>

Result language

angličtina

Original language name

MicroRNA dilution during oocyte growth disables the microRNA pathway in mammalian oocytes

Original language description

MicroRNAs (miRNAs) are ubiquitous small RNAs guiding post-transcriptional gene repression in countless biological processes. However, the miRNA pathway in mouse oocytes appears inactive and dispensable for development. We propose that marginalization of the miRNA pathway activity stems from the constraints and adaptations of RNA metabolism elicited by the diluting effects of oocyte growth. We report that miRNAs do not accumulate like mRNAs during the oocyte growth because miRNA turnover has not adapted to it. The most abundant miRNAs total tens of thousands of molecules in growing (empty set 40 mu m) and fully grown (empty set 80 mu m) oocytes, a number similar to that observed in much smaller fibroblasts. The lack of miRNA accumulation results in a 100-fold lower miRNA concentration in fully grown oocytes than in somatic cells. This brings a knockdown-like effect, where diluted miRNAs engage targets but are not abundant enough for significant repression. Low-miRNA concentrations were observed in rat, hamster, porcine and bovine oocytes, arguing that miRNA inactivity is not mouse-specific but a common mammalian oocyte feature. Injection of 250,000 miRNA molecules was sufficient to restore reporter repression in mouse and porcine oocytes, suggesting that miRNA inactivity comes from low-miRNA abundance and not from some suppressor of the pathway.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

48

Issue of the periodical within the volume

14

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

8050-8062

UT code for WoS article

000574297400038

EID of the result in the Scopus database

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