Physiologically relevant miRNAs in mammalian oocytes are rare and highly abundant
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00554909" target="_blank" >RIV/67985904:_____/22:00554909 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/22:00554909
Result on the web
<a href="https://www.embopress.org/doi/full/10.15252/embr.202153514" target="_blank" >https://www.embopress.org/doi/full/10.15252/embr.202153514</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15252/embr.202153514" target="_blank" >10.15252/embr.202153514</a>
Alternative languages
Result language
angličtina
Original language name
Physiologically relevant miRNAs in mammalian oocytes are rare and highly abundant
Original language description
miRNAs, similar to 22nt small RNAs associated with Argonaute (AGO) proteins, are important negative regulators of gene expression in mammalian cells. However, mammalian maternal miRNAs show negligible repressive activity and the miRNA pathway is dispensable for oocytes and maternal-to-zygotic transition. The stoichiometric hypothesis proposed that this is caused by dilution of maternal miRNAs during oocyte growth. As the dilution affects miRNAs but not mRNAs, it creates unfavorable miRNA:mRNA stoichiometry for efficient repression of cognate mRNAs. Here, we report that porcine ssc-miR-205 and bovine bta-miR-10b are exceptional miRNAs, which resist the diluting effect of oocyte growth and can efficiently suppress gene expression. Additional analysis of ssc-miR-205 shows that it has higher stability, reduces expression of endogenous targets, and contributes to the porcine oocyte-to-embryo transition. Consistent with the stoichiometric hypothesis, our results show that the endogenous miRNA pathway in mammalian oocytes is intact and that maternal miRNAs can efficiently suppress gene expression when a favorable miRNA:mRNA stoichiometry is established.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Embo Reports
ISSN
1469-221X
e-ISSN
1469-3178
Volume of the periodical
23
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
e53514
UT code for WoS article
000726612700001
EID of the result in the Scopus database
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